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Application of molecular biotechnology to improve biodegradation of anaerobic/aerobic membrane bioreactor

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Characterization of the TFT-LCD manufacturing wastewater discharged from three plants was conducted. Relatively high ratio of organic nitrogen to organic carbon conducted from certain amount of stripper (DMSO&MEA) developer (TMAH) and chelating agents. So tow stages of anoxic denitrification and oxic nitrification processes are developed to degrade the specific organic nitrogen and ammonium. Non-woven membrane bioreactors (NW-MBR) are designed and installed with plate-frame modules of Non-woven membrane, so called KNH-MBR.Tow months of continuous flow process of tow-stage KNH-MBR have been studied to evaluate the performance of denitrification and nitrification. The anoxic MBR attended to limited degradation of organic nitrogen and denitrification, while the aerobic MBR achieved good removal efficiency of TOC COD organic nitrogen and ammonium. Extended process operation should be done with acclimated inoculation and enough biomass of denitrifier.Four methods of biodegradation batch test are conducted. The biochemical hydrogen potential (BHP) test, the BMP test, the BNP test and the specific oxygen uptake rate (SOUR) measurement showed the KNH-MBR anaerobic and aerobes degraded TOC, organic nitrogen and ammonium oxidation with nitrifier.The ecological indicators of various function bacteria were observed with the scanning electronic microscopy. Terminal restriction fragment length polymorphism (T-RFLP) was modified and developed for identification of nitrifier and denitrifier in the anoxic sludge and oxic sludge of LCD wastewater treatment plants fingerprint of bacterial species could be expressed from the T.RFLP diagram. Several species of ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were identified according to the genetic database. So molecular bio monitoring methodology could be conducted to investigate the population dynamics of the existed microbial community in the MBR process.
Keyword
TFT-LCD wastewater,membrane bioreactor (MBR),Terminal restriction fragment length polymorphism (T-RFLP),microbial diversity
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